Epitope-Based Vaccine Target Screening against Highly Pathogenic MERS-CoV: An In Silico Approach Applied to Emerging Infectious Diseases
Identifieur interne : 001805 ( Main/Exploration ); précédent : 001804; suivant : 001806Epitope-Based Vaccine Target Screening against Highly Pathogenic MERS-CoV: An In Silico Approach Applied to Emerging Infectious Diseases
Auteurs : Jiandong Shi [République populaire de Chine] ; Jing Zhang [République populaire de Chine] ; Sijin Li [République populaire de Chine] ; Jing Sun [République populaire de Chine] ; Yumei Teng [République populaire de Chine] ; Meini Wu [République populaire de Chine] ; Jianfan Li [République populaire de Chine] ; Yanhan Li [République populaire de Chine] ; Ningzhu Hu [République populaire de Chine] ; Haixuan Wang [République populaire de Chine] ; Yunzhang Hu [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Descripteurs français
- KwdFr :
- Anticorps neutralisants (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Déterminants antigéniques des lymphocytes B (immunologie), Déterminants antigéniques des lymphocytes T (immunologie), Glycoprotéine de spicule des coronavirus (immunologie), Humains, Immunité cellulaire (immunologie), Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Lymphocytes T auxiliaires (immunologie), Protéines nucléocapside (immunologie), Vaccins antiviraux (immunologie).
- MESH :
- immunologie : Anticorps neutralisants, Coronavirus du syndrome respiratoire du Moyen-Orient, Déterminants antigéniques des lymphocytes B, Déterminants antigéniques des lymphocytes T, Glycoprotéine de spicule des coronavirus, Immunité cellulaire, Infections à coronavirus, Lymphocytes T auxiliaires, Protéines nucléocapside, Vaccins antiviraux.
- virologie : Infections à coronavirus.
- Humains.
English descriptors
- KwdEn :
- Antibodies, Neutralizing (immunology), Coronavirus Infections (immunology), Coronavirus Infections (virology), Epitopes, B-Lymphocyte (immunology), Epitopes, T-Lymphocyte (immunology), Humans, Immunity, Cellular (immunology), Middle East Respiratory Syndrome Coronavirus (immunology), Nucleocapsid Proteins (immunology), Spike Glycoprotein, Coronavirus (immunology), T-Lymphocytes, Helper-Inducer (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , immunology : Antibodies, Neutralizing, Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, Nucleocapsid Proteins, Spike Glycoprotein, Coronavirus, Viral Vaccines.
- immunology : Coronavirus Infections, Immunity, Cellular, Middle East Respiratory Syndrome Coronavirus, T-Lymphocytes, Helper-Inducer.
- virology : Coronavirus Infections.
- Humans.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) with pandemic potential is a major worldwide threat to public health. However, vaccine development for this pathogen lags behind as immunity associated with protection is currently largely unknown. In this study, an immunoinformatics-driven genome-wide screening strategy of vaccine targets was performed to thoroughly screen the vital and effective dominant immunogens against MERS-CoV. Conservancy and population coverage analysis of the epitopes were done by the Immune Epitope Database. The results showed that the nucleocapsid (N) protein of MERS-CoV might be a better protective immunogen with high conservancy and potential eliciting both neutralizing antibodies and T-cell responses compared with spike (S) protein. Further, the B-cell, helper T-cell and cytotoxic T lymphocyte (CTL) epitopes were screened and mapped to the N protein. A total of 15 linear and 10 conformal B-cell epitopes that may induce protective neutralizing antibodies were obtained. Additionally, a total of 71 peptides with 9-mer core sequence were identified as helper T-cell epitopes, and 34 peptides were identified as CTL epitopes. Based on the maximum HLA binding alleles, top 10 helper T-cell epitopes and CTL epitopes that may elicit protective cellular immune responses against MERS-CoV were selected as MERS vaccine candidates. Population coverage analysis showed that the putative helper T-cell epitopes and CTL epitopes could cover the vast majority of the population in 15 geographic regions considered where vaccine would be employed. The B- and T-cell stimulation potentials of the screened epitopes is to be further validated for their efficient use as vaccines against MERS-CoV. Collectively, this study provides novel vaccine target candidates and may prompt further development of vaccines against MERS-CoV and other emerging infectious diseases.
Url:
DOI: 10.1371/journal.pone.0144475
PubMed: 26641892
PubMed Central: 4671582
Affiliations:
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first="Jiandong" last="Shi">Jiandong Shi</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Shijiazhuang, Hebei</wicri:regionArea><wicri:noRegion>Hebei</wicri:noRegion></affiliation></author><author><name sortKey="Li, Sijin" sort="Li, Sijin" uniqKey="Li S" first="Sijin" last="Li">Sijin Li</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Jing" sort="Sun, Jing" uniqKey="Sun J" first="Jing" last="Sun">Jing Sun</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Teng, Yumei" sort="Teng, Yumei" uniqKey="Teng Y" first="Yumei" last="Teng">Yumei Teng</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Meini" sort="Wu, Meini" uniqKey="Wu M" first="Meini" last="Wu">Meini Wu</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Li, Jianfan" sort="Li, Jianfan" uniqKey="Li J" first="Jianfan" last="Li">Jianfan Li</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yanhan" sort="Li, Yanhan" uniqKey="Li Y" first="Yanhan" last="Li">Yanhan Li</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Ningzhu" sort="Hu, Ningzhu" uniqKey="Hu N" first="Ningzhu" last="Hu">Ningzhu Hu</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Haixuan" sort="Wang, Haixuan" uniqKey="Wang H" first="Haixuan" last="Wang">Haixuan Wang</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Yunzhang" sort="Hu, Yunzhang" uniqKey="Hu Y" first="Yunzhang" last="Hu">Yunzhang Hu</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Yunnan Provincial Key Laboratory of Arbo Infectious Disease Control Research (Preparing), Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming</wicri:regionArea><wicri:noRegion>Kunming</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Neutralizing (immunology)</term><term>Coronavirus Infections (immunology)</term><term>Coronavirus Infections (virology)</term><term>Epitopes, B-Lymphocyte (immunology)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>Humans</term><term>Immunity, Cellular (immunology)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Nucleocapsid Proteins (immunology)</term><term>Spike Glycoprotein, Coronavirus (immunology)</term><term>T-Lymphocytes, Helper-Inducer (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps neutralisants (immunologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Déterminants antigéniques des lymphocytes B (immunologie)</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains</term><term>Immunité cellulaire (immunologie)</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (virologie)</term><term>Lymphocytes T auxiliaires (immunologie)</term><term>Protéines nucléocapside (immunologie)</term><term>Vaccins antiviraux (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term><term>Epitopes, B-Lymphocyte</term><term>Epitopes, T-Lymphocyte</term><term>Nucleocapsid Proteins</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps neutralisants</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Déterminants antigéniques des lymphocytes B</term><term>Déterminants antigéniques des lymphocytes T</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunité cellulaire</term><term>Infections à coronavirus</term><term>Lymphocytes T auxiliaires</term><term>Protéines nucléocapside</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term><term>Immunity, Cellular</term><term>Middle East Respiratory Syndrome Coronavirus</term><term>T-Lymphocytes, Helper-Inducer</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) with pandemic potential is a major worldwide threat to public health. However, vaccine development for this pathogen lags behind as immunity associated with protection is currently largely unknown. In this study, an immunoinformatics-driven genome-wide screening strategy of vaccine targets was performed to thoroughly screen the vital and effective dominant immunogens against MERS-CoV. Conservancy and population coverage analysis of the epitopes were done by the Immune Epitope Database. The results showed that the nucleocapsid (N) protein of MERS-CoV might be a better protective immunogen with high conservancy and potential eliciting both neutralizing antibodies and T-cell responses compared with spike (S) protein. Further, the B-cell, helper T-cell and cytotoxic T lymphocyte (CTL) epitopes were screened and mapped to the N protein. A total of 15 linear and 10 conformal B-cell epitopes that may induce protective neutralizing antibodies were obtained. Additionally, a total of 71 peptides with 9-mer core sequence were identified as helper T-cell epitopes, and 34 peptides were identified as CTL epitopes. Based on the maximum HLA binding alleles, top 10 helper T-cell epitopes and CTL epitopes that may elicit protective cellular immune responses against MERS-CoV were selected as MERS vaccine candidates. Population coverage analysis showed that the putative helper T-cell epitopes and CTL epitopes could cover the vast majority of the population in 15 geographic regions considered where vaccine would be employed. The B- and T-cell stimulation potentials of the screened epitopes is to be further validated for their efficient use as vaccines against MERS-CoV. Collectively, this study provides novel vaccine target candidates and may prompt further development of vaccines against MERS-CoV and other emerging infectious diseases.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bermingham, A" uniqKey="Bermingham A">A Bermingham</name></author><author><name sortKey="Chand, Ma" uniqKey="Chand M">MA Chand</name></author><author><name sortKey="Brown, Cs" uniqKey="Brown C">CS Brown</name></author><author><name sortKey="Aarons, E" uniqKey="Aarons E">E Aarons</name></author><author><name sortKey="Tong, C" uniqKey="Tong C">C Tong</name></author><author><name sortKey="Langrish, C" uniqKey="Langrish C">C Langrish</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer 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uniqKey="Hu Y" first="Yunzhang" last="Hu">Yunzhang Hu</name><name sortKey="Li, Jianfan" sort="Li, Jianfan" uniqKey="Li J" first="Jianfan" last="Li">Jianfan Li</name><name sortKey="Li, Jianfan" sort="Li, Jianfan" uniqKey="Li J" first="Jianfan" last="Li">Jianfan Li</name><name sortKey="Li, Sijin" sort="Li, Sijin" uniqKey="Li S" first="Sijin" last="Li">Sijin Li</name><name sortKey="Li, Sijin" sort="Li, Sijin" uniqKey="Li S" first="Sijin" last="Li">Sijin Li</name><name sortKey="Li, Yanhan" sort="Li, Yanhan" uniqKey="Li Y" first="Yanhan" last="Li">Yanhan Li</name><name sortKey="Li, Yanhan" sort="Li, Yanhan" uniqKey="Li Y" first="Yanhan" last="Li">Yanhan Li</name><name sortKey="Shi, Jiandong" sort="Shi, Jiandong" uniqKey="Shi J" first="Jiandong" last="Shi">Jiandong Shi</name><name sortKey="Sun, Jing" sort="Sun, Jing" uniqKey="Sun J" first="Jing" last="Sun">Jing Sun</name><name sortKey="Sun, Jing" sort="Sun, Jing" uniqKey="Sun J" first="Jing" last="Sun">Jing Sun</name><name sortKey="Teng, Yumei" sort="Teng, Yumei" uniqKey="Teng Y" first="Yumei" last="Teng">Yumei Teng</name><name sortKey="Teng, Yumei" sort="Teng, Yumei" uniqKey="Teng Y" first="Yumei" last="Teng">Yumei Teng</name><name sortKey="Wang, Haixuan" sort="Wang, Haixuan" uniqKey="Wang H" first="Haixuan" last="Wang">Haixuan Wang</name><name sortKey="Wang, Haixuan" sort="Wang, Haixuan" uniqKey="Wang H" first="Haixuan" last="Wang">Haixuan Wang</name><name sortKey="Wu, Meini" sort="Wu, Meini" uniqKey="Wu M" first="Meini" last="Wu">Meini Wu</name><name sortKey="Wu, Meini" sort="Wu, Meini" uniqKey="Wu M" first="Meini" last="Wu">Meini Wu</name><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name><name sortKey="Zhang, Jing" sort="Zhang, Jing" uniqKey="Zhang J" first="Jing" last="Zhang">Jing Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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